Molecular architecture of coronavirus double-membrane vesicle pore complex
Published:
Publishing date: 2024-02-19
Journal: Nature
Institution: Hong Kong University
Authors: Yixin Huang, Tongyun Wang, Lijie Zhong, Wenxin Zhang, Yu Zhang, Xiulian Yu, Shuofeng Yuan, and Tao Ni
Method
Cryo-ET STA
Summary
SARS-CoV-2 nsp3 and nsp4 can induce the formation of double-membrane vesicles to package viral RNA inside. They purified the DMVs using a TwinStrep-EGFP tag on nsp3 by transfecting Expi293F cells. They collected 4,000 tomograms and obtained the pore complex structure using subtomogram averaging (STA). The structure looks nice, but I believe single-particle analysis would be a better approach for solving this structure.